What is amplification refractory mutation system?

The amplification-refractory mutation system (ARMS) is a simple method for detecting any mutation involving single base changes or small deletions. ARMS is based on the use of sequence-specific PCR primers that allow amplification of test DNA only when the target allele is contained within the sample.

What is ASA PCR?

Recently, allele specific amplification (ASA) by the amplification refractory mutation system (ARMS) has been introduced, avoiding the use of restriction enzymes and radioisotopes (1). This method requires two separate polymerase chain reactions (PCR) to amplify each allele using different primers.

What is multiplex PCR used for?

Multiplex PCR methods are usually used for the simultaneous detection of two or more target pathogenic DNA or RNA molecules by using several specific primers in a single PCR reaction.

What is endpoint PCR?

End point PCR is the analysis after all cycles of PCR are completed. Unlike qPCR, which allows quantification as template is doubling (exponential phase), end point analysis is based on the platau phase of amplification. The quantification is made by densitometry after exposure to a Xray film.

What is SSP PCR?

Sequence-specific amplification (SSP) is simply a form of polymerase chain reaction (PCR) which involves designing one or both primers so that they will or will not allow amplification (the 3′-mismatch principle).

What is the difference between a Singleplex and a multiplex PCR?

In conventional singleplex PCR, a single target is amplified in a single reaction tube. In contrast, multiplex PCR allows for simultaneous amplification of multiple target sequences in a single tube using specific primer sets in combination with probes labeled with spectrally distinct fluorophores.

How many primers are used in PCR?

Two primers
Two primers are used in each PCR reaction, and they are designed so that they flank the target region (region that should be copied). That is, they are given sequences that will make them bind to opposite strands of the template DNA, just at the edges of the region to be copied.

What is gradient PCR?

Gradient PCR is a technique that allows the empirical determination of an optimal annealing temperature using the least number of steps. The Eppendorf Mastercycler Gradient provides a gradient function that in one single run evaluates up to 12 different annealing, elongation, or denaturation temperatures.