What is attB?
attB: Stands for bacterial attachment site which helps in site specific recombination and always attaches with the PCR product. It consist of four homologous core sequence of ~25 bp each, attB1 ACAAGTTTGTACAAAAAAGCAGGCT.
What is a destination vector?
Gateway Destination vectors are similar to classical expression vectors containing multiple cloning sites, before the insertion of a gene of interest, using restriction enzyme digestion and ligation. Gateway Destination vectors are commercially available from Invitrogen, EMD (Novagen) and Covalys.
What is a gateway vector?
Gateway vectors contain modified versions of the att sites so that scientists can easily clone in their desired DNA sequences. Gateway technology relies on the two reactions described below: The BP Reaction takes place between the attB sites flanking the insert and the attP sites of the donor vector.
What is recombination cloning?
Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence to be cloned, which differentiates this method from the classical restriction enzyme-based cloning methods.
What is Gateway Destination?
A tourist gateway (sometimes called a “Tourism Gateway” or “Gateway City”) is a place or settlement through which tourists typically first visit on their way to a tourist attraction or tourism region. Tourist gateways may not offer significant attractions themselves.
Is recombinant DNA the same as cloning?
Recombinant molecules enter living cells in a process called transformation. Each colony is a cell clone, but it is also a DNA clone because the recombinant vector has now been amplified by replication during every round of cell division.
What is the difference between attb1 and attb2 and attl2?
They have modified versions of the attB, P, L and R sites that recombine very specifically and directionally: attB1 sites react only with attP1 sites; attB2 only with attP2, attL1 only with attR1; attL2 only with attR2, and so on.
What is vectorvector database?
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only – this vector is NOT available from Addgene – please contact the manufacturer for further details.
What is the difference between the puastattb vector and the donor vector?
One vector referred to as the pUASTattB vector or the φC31 donor vector carries the attB site and the user’s gene of interest. The other vector referred to as the helper plasmid encodes for the φC31 integrase.
What happens when the attL and ATTR sites recombine?
Under certain conditions, the attL and attR sites can recombine, leading to the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. Figure 1: Lambda phage integration and excision reactions. Recombination of attP and attB sites creates attL and attR sites.
