What is plasmid mapping in lab 12?

LAB 12 PLASMID MAPPING STUDENT GUIDE GOAL The objective of this lab is to perform restriction digestion of plasmid DNA and construct a plasmid map using the results. OBJECTIVES After completion, the student should be able to: 1. Perform plasmid DNA mapping. 2. Draw a plasmid map from gel electrophoresis data of the digested recombinant plasmid.

How do I view plasmid annotated files?

Your time is valuable! Our software is designed to save you time. As part of that effort, we supply carefully annotated files for common plasmids. Click on the links to view the plasmid collections. These combined DNA sequence and map files can be opened with SnapGene or the free SnapGene Viewer.

How many restriction sites are there in a plasmid?

These data show that each enzyme has only one restriction site within the plasmid. Without further experimentation, there is no way to know where each of the three sites is located with respect to the other two. Therefore, double and even triple digestions are performed, run on a gel, and analyzed.

What is the objective of the plasmid DNA lab?

The objective of this lab is to perform restriction digestion of plasmid DNA and construct a plasmid map using the results. OBJECTIVES After completion, the student should be able to: 1. Perform plasmid DNA mapping. 2. Draw a plasmid map from gel electrophoresis data of the digested recombinant plasmid. 3.

Which plasmid to use for TCF/LEF binding?

The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites. This construct was made by Ajamete Kaykas in the Moon lab.

How to quantify Wnt / Wg signaling in cells transfected with fopflash?

The Tcf reporter plasmid and FOPFLASH can quantify Wnt / Wg signaling in cells transfected with these constructs. Transfection grade T cell factor (Tcf) reporter plasmid containing three copies of the Tcf binding site (wt) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame.